Selective removal of keratan sulfate in chondroitin sulfate samples by sequential precipitation with ethanol
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- 作者：Fabio Galeotti ; Francesca Maccari ; Nicola Volpi
- 关键词：Chondroitin sulfate ; Keratan sulfate ; Ethanol precipitation ; Hexosamines ; Capillary electrophoresis ; Glycosaminoglycans
- 刊名：Analytical Biochemistry
- 出版年：1 March, 2014
- 年：2014
- 卷：448
- 期：Complete
- 页码：113-115
- 全文大小：546 K
文摘
Keratan sulfate (KS) is present as a contaminant in chondroitin sulfate (CS) mainly extracted from shark cartilage. We report a selective removal procedure of KS in CS samples by means of sequential precipitation with ethanol. Purified shark CS containing approximately 10% to 15% KS was subjected to a precipitation procedure in the presence of increasing percentages of saturated ethanol. In contrast to other solvents, 1.0 volume of ethanol was able to selectively purify CS, with a purity of approximately 100%, from KS. The current selective and simple procedure appears to be a reliable industrial preparation of CS devoid of large amounts of the residual KS.