Characterization of Trypanosoma cruzi telomerase

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• 作者：Riward  ; Campelo ; Maria Mercedes  ; Galindo ; Jose Luis  ; Ramirez  ; ;   ; ^{ramjoseluis@gmail.com} ;   ; ^{jramirez@idea.gob.ve}
• 关键词：Trypanosoma cruzi ; Heat-activated telomerase ; Telomerase inhibitors ; Biochemical characterization ; Non-radioactive TRAP assay
• 刊名：Acta Tropica
• 出版年：2011
• 出版时间：December 2011
• 年：2011
• 卷：120
• 期：3
• 页码：173-178
• 全文大小：843 K

文摘

High telomerase activity is always associated with actively dividing cells, however the detection of this activity in dividing Leishmania and Trypanosoma cruzi cells has always been disappointingly low. Recently, we have found that Leishmania major telomerase activity can be activated by heat, which combined with dilutions of the nuclear extracts produced an increase in activity comparable to cancer cells. Here we examined whether T. cruzi telomerase shares the same physicochemical properties of primer specificity and overall features of the L. major.

Our studies revealed that no telomerase inhibitory factors were present in the nuclear lysates of T. cruzi however the enzyme was activated by heat and was very resilient to heat denaturation. We also showed the extension primer specificity, susceptibility to RNase-A and RNase-H digestion, and the effect of telomerase inhibitors.