Detection of Epstein-Barr Virus and Genotyping Based on EBNA2 Protein in Mexican Patients With Hodgkin Lymphoma: A Comparative Study in Children and Adults

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• 作者：Icela Palma¹ ; ² ; Ana Elena Sá ; nchez¹ ; ³ ; Elva Jimé ; nez-Herná ; ndez⁴ ; ⁵ ; Francisco Alvarez-Rodrí ; guez⁴ ; Margarita Nava-Frias³ ; Pedro Valencia-Mayoral⁶ ; Citlatepet Salinas-Lara⁷ ; Norma Velazquez-Guadarrama³ ; Josefina Portilla-Aguilar⁴ ; Rocio Y. Pena⁸ ; Patricia Ramos-Salazar⁹ ; Alejandra Contreras¹⁰ ; Ana Alfaro¹¹ ; Ana Maria Espinosa¹¹ ; Nayelli Ná ; jera¹ ; Gisela Gutierrez¹² ; Juan Manuel Mejia-Arangure¹³ ; José ; Arellano-Galindo³ ; ^{jose.arellano@salud.gob.mx}
• 关键词：DNA ; EBER ; EBNA2A ; EBNA2B ; Epstein-Barr virus ; In situ hybridization ; LMP
• 刊名：Clinical Lymphoma Myeloma and Leukemia
• 出版年：2013
• 出版时间：June, 2013
• 年：2013
• 卷：13
• 期：3
• 页码：266-272
• 全文大小：1177 K

文摘

Background

Epstein-Barr virus (EBV) is a member of the Herpesviridae family and is associated with Hodgkin lymphoma (HL). Isolates of EBV are classified according to sequence variation in the latency genes such as Epstein-Barr virus nuclear antigen (EBNA). EBNA2 contains the most divergent locus and is classified into type 1 and type 2 or EBNA2A and EBNA2B, respectively. We compared the frequency of EBV and the distribution of EBNA genotypes in Mexican children and adults with HL.

Patients and Methods

Lymph node biopsy specimens from children and adults with HL were embedded in paraffin. EBV was identified by LMP1 amplification and Epstein-Barr-encoded RNA EBER by in situ hybridization (ISH) and genotyped as EBNA2A or EBNA2B using nested polymerase chain reaction (PCR) and specific primers for the detection of subtype.

Results

Sixty-six samples were obtained from 3 hospitals¡ª42 (63 % ) from children and 24 (37 % ) from adults with HL. Thirty-two of the 42 samples (76.1 % ) were positive for EBV in children and 16 of 24 (66.6 % ) samples were positive in adults (P = .41). In both children and adults, EBV was found more frequently in male patients. Thirty-four of 48 cases could be typed (70.8 % ). EBNA2A was found in 7/21 (33.3 % ) children and in 4/13 (30.8 % ) adults (P = 1.0), and EBNA2B was found in 10/21 (47.6 % ) children and in 9/13 (69.2 % ) adults (P = .22). A mix of subtypes was found in 4/21 (19 % ) children.

Conclusion

EBV was found frequently in both children and adults with HL. EBNA2B was the most frequent subtype, and a high frequency of mixed subtypes was found in children.