Vitellogenin induction in the endangered goodeid fish Girardinichthys viviparus: Vitellogenin characterization and estrogenic effects of polychlorinated biphenyls

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• 作者：Armando Vega-Ló ; pez ; Laura Martí ; nez-Tabche ; Maria Lilia Domí ; nguez-Ló ; pez ; Ethel Garcí ; a-Latorre ; Eva Ramó ; n-Gallegos and Alejandra Garcí ; a-Gasca
• 关键词：Endocrine disruption ; Girardinichthys viviparus ; Goodeid fish ; Vitellogenin ; PCBs ; Waterborne exposure ; Sex differences
• 刊名：Comparative Biochemistry and Physiology, Part C
• 出版年：2006
• 出版时间：March-April 2006
• 年：2006
• 卷：142
• 期：3-4
• 页码：356-364
• 全文大小：197 K

文摘

Vitellogenin (VTG) is a widely used biomarker in studies of endocrine disruption induced by xenobiotics such as polychlorinated biphenyls (PCBs). This study evaluates the estrogenic effects of these compounds on the black-fin goodeid Girardinichthys viviparus, an endangered fish species in Mexico with a reduced range of distribution due to pollution of its natural environment. Adult fish born in the laboratory were exposed to half the LC₀ of Inerteen® commercial PCB mixture. VTG was determined through an inhibition enzyme-linked immunosorbent assay (ELISA) using a homologous–heterologous system. Male and female fish were killed after 1, 2, 4, 8 and 16 days of exposure. The distal third of each specimen was used for analysis. VTG was obtained from cultured hepatocytes and blood serum of males previously exposed to 17β-estradiol. VTG molecular mass was 348 kDa. PCBs were found to elicit greater estrogenic effects on VTG induction in males than in females (p < 0.05) and sex differences were noted. Time-dependent VTG induction kinetics in males and a stationary phase in females were also observed.