Immunoprotective activities of a Streptococcus suis pilus subunit in murine models of infection
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- 作者：Manuela Garibaldi ; Manuel J. Rodrí ; guez-Ortega ; Francesca M ; anici ; Angela Cardaci ; Angelina Midiri ; Salvatore Papasergi ; Orazio Gambadoro ; Vittorio Cavallari ; Giuseppe Teti ; Concetta Beninati
- 关键词：Streptococcus suis ; Pili ; Proteomics
- 刊名：Vaccine
- 出版年：2010
- 出版时间：30 April 2010
- 年：2010
- 卷：28
- 期：20
- 页码：3609-3616
- 全文大小：1295 K
文摘
Pili of gram-positive bacteria are key virulence factors and their subunits are considered excellent vaccine candidates. Streptococcus suis is an emerging zoonotic agent that can cause epidemics of life-threatening infections in humans, but the functional role or immunoprotective potential of its pilus components have not been studied yet. Using a selective proteomics approach, we have identified a surface protein of serotype 2 S. suis showing features of an ancillary pilus subunit, as evidenced by bioinformatics analysis, immunoblot and immunoelectron microscopy. Immunization with recombinant fragments of this protein, designated herein as PAPI-2b, markedly protected mice from systemic S. suis infection.