Bone marrow stem cells assuage radiation-induced damage in a murine model of distraction osteogenesis: A histomorphometric evaluation

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• 作者：Alexander R. Zheutlin¹ ; Sagar S. Deshpande¹ ; Noah S. Nelson¹ ; Stephen Y. Kang¹ ; ² ; Kathleen K. Gallagher¹ ; ³ ; Yekaterina Polyatskaya¹ ; Jose J. Rodriguez¹ ; Alexis Donneys¹ ; Kavitha Ranganathan¹ ; Steven R. Buchman¹ ; ^{sbuchman@med.umich.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：histology ; mandible ; osteocyte ; radiotherapy
• 刊名：Cytotherapy
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：18
• 期：5
• 页码：664-672
• 全文大小：1488 K

文摘

The purpose of this study is to determine if intraoperatively placed bone marrow stem cells (BMSCs) will permit successful osteocyte and mature bone regeneration in an isogenic murine model of distraction osteogenesis (DO) following radiation therapy (XRT). Lewis rats were split into three groups, DO only (Control), XRT followed by DO (xDO) and XRT followed by DO with intraoperatively placed BMSCs (xDO-BMSC). Coronal sections from the distraction site were obtained, stained and analyzed via statistical analysis with analysis of variance (ANOVA) and subsequent Tukey or Games-Howell post-hoc tests. Comparison of the xDO-BMSC and xDO groups demonstrated significantly improved osteocyte count (87.15 ± 10.19 vs. 67.88 ± 15.38, P = 0.00), and empty lacunae number (2.18 ± 0.79 vs 12.34 ± 6.61, P = 0.00). Quantitative analysis revealed a significant decrease in immature osteoid volume relative to total volume (P = 0.00) and improved the ratio of mature woven bone to immature osteoid (P = 0.02) in the xDO-BMSC compared with the xDO group. No significant differences were found between the Control and xDO-BMSC groups. In an isogenic murine model of DO, BMSC therapy assuaged XRT-induced cellular depletion, resulting in a significant improvement in histological and histomorphometric outcomes.