Effects of lipopolysaccharide exposure at different postnatal time points on the response of LH to homotypic stress in adulthood

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• 作者：Takeshi Iwasa ; Toshiya Matsuzaki ; ^{mat@clin.med.tokushima-u.ac.jp} ; Masahiro Murakami ; Riyo Kinouchi ; Ganbat Gereltsetseg ; Hiroshi Nakazawa ; Satoshi Yamamoto ; Akira Kuwahara ; Toshiyuki Yasui ; Minoru Irahara
• 关键词：LPS ; LH ; IL-1¦Â ; TNF-¦Á ; Critical window
• 刊名：Journal of Reproductive Immunology
• 出版年：2012
• 出版时间：June, 2012
• 年：2012
• 卷：94
• 期：2
• 页码：155-160
• 全文大小：436 K

文摘

Early-life immune stress may have long-lasting effects, known as programming effects, on the physiological response to stress in adulthood. There may be a critical window after birth during which such a challenge can induce long-lasting alterations. However, there are few reports regarding the consequences of this phenomenon for later reproductive function. Here we report on induction by early-life LPS injection of long-lasting alterations in the adult LH response to homotypic immune stress in male rats. First, we investigated developmental changes in the LH response to LPS, since immune challenge during the stress hyporesponsive period can induce long-lasting effects on physiological functions. Rat serum LH concentrations were decreased by LPS (100 ¦Ìg/kg) injection on postnatal day 15 or 25, but not day 10, suggesting that the period prior to postnatal day 10 is the stress hyporesponsive period for LH. Serum LH concentrations and body weight were decreased by adult LPS (400 ¦Ìg/kg) injection in rats given saline or LPS (100 ¦Ìg/kg) on postnatal day 25, but not in rats given LPS (100 ¦Ìg/kg) on postnatal day 10. Expression of hypothalamic IL-1¦Â and TNF-¦Á mRNA, which suppress serum LH during immune stress, were equally increased in these groups by adult LPS (400 ¦Ìg/kg) injection. The present data suggest that the period prior to postnatal day 10 is the critical window in which immune stress can induce long-lasting alterations in the LH response, but that IL-1¦Â and TNF-¦Á are not involved in mediating the altered response.