Solubility, inhibition of crystallization and microscopic analysis of calcium oxalate crystals in the presence of fractions from Humulus lupulus L.

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• 作者：Anna Frą ; ckowiak ; Tomasz Koź ; lecki ; PrzemysŁ ; aw Skibiń ; ski ; WiesŁ ; aw GaweŁ ; Ewa Zaczyń ; ska ; Anna Czarny ; Katarzyna Piekarska ; Roman Gancarz
• 关键词：A1. Biocrystallization ; A1. Crystal morphology ; A1. Dissolution of calcium oxalate ; A1. Optical microscopy ; A2. Inhibition of crystal growth ; B1. Humulus lupulus L.
• 刊名：Journal of Crystal Growth
• 出版年：2010
• 出版时间：15 November 2010
• 年：2010
• 卷：312
• 期：23
• 页码：3525-3532
• 全文大小：762 K

文摘

Procedures for obtaining noncytotoxic and nonmutagenic extracts from Humulus lupulus L. of high potency for inhibition and dissolving of model (calcium oxalate crystals) and real kidney stones, obtained from patients after surgery, are presented. Multistep extraction procedures were performed in order to obtain the preparations with the highest calcium complexing properties. The composition of obtained active fractions was analyzed by GC/MS and NMR methods. The influence of preparations on inhibition of formation and dissolution of model and real kidney stones were evaluated based on conductrometric titration, flame photometry and microscopic analysis.

The “fraction soluble in methanol” obtained from water–alkaline extracts contains sugar alcohols and organic acids, and is effective in dissolving the kidney stones. The “fraction insoluble in methanol” contains only sugar derivatives and it changes the morphology of the crystals, making them “jelly-like”. Both fractions are potentially effective in kidney stone therapy.