Comparison of the immunomodulatory effects of L. donovani

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• 作者：Matthieu Revest ; Ludovic Donaghy ; Florian Cabillic ; Claude Guiguen ; Jean-Pierre Gangneux
• 关键词：Leishmania ; Excreted– ; secreted antigens ; Dendritic cells
• 刊名：Vaccine
• 出版年：2008
• 出版时间：11 November 2008
• 年：2008
• 卷：26
• 期：48
• 页码：6119-6123
• 全文大小：448 K

文摘

In an experimental model of human monocyte-derived dendritic cells (DCs), the immunophenotype of mature DCs infected with Leishmania donovani and Leishmania major showed a weak decrease in the cell surface expression of CD40, CD86, HLA-DR and DC-SIGN compared with uninfected control DCs. This immunomodulatory effect was more pronounced after stimulation with excreted–secreted antigens (ESA) of both species but absent after stimulation with particulate and soluble extracts. Infection with viable promastigotes, as well as stimulation with ESA from L. donovani and L. major, decreased IL-10 and IL-12p70 secretion. To our knowledge, this is the first direct demonstration that ESA from Leishmania promastigotes can stimulate DCs in the same manner as viable promastigotes.