Electrochemical lectin-based biosensor array for detection and discrimination of carcinoembryonic antigen using dual amplification of gold nanoparticles and horseradish peroxidase
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- 作者：Lingzhi ZhaoAuthor Vitae ; Congcong Li1Author Vitae ; Honglan Qi ; honglanqi@snnu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; honglanqi@hotmail.com" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Qiang GaoAuthor Vitae ; Chengxiao ZhangAuthor Vitae
- 关键词：Electrochemical biosensor ; Array ; Lectin ; Carcinoembryonic antigen ; Amplification
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2016
- 出版时间：1 November 2016
- 年：2016
- 卷：235
- 期：Complete
- 页码：575-582
- 全文大小：2040 K
文摘
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An electrochemical biosensor for the determination of carcinoembryonic antigen was developed.
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Lectins including wheat-germ agglutinin, Lens culinaris agglutinin, concanavalin A were employed as molecular recognition elements.
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The detection limit for CEA was 0.03 ng/mL, 0.05 ng/mL, and 0.01 ng/mL using Con A, WGA, and LCA as molecular recognition elements, respectively.
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A lectin-based biosensor array was used to evaluate the glycan expression of CEA N-glycan.
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A biosensor array was used to discriminate CEA between healthy and cancer serum samples.