Efficient gene transfection in the neurotypic cells by star-shaped polymer consisting of ¦Â-cyclodextrin core and poly(amidoamine) dendron arms

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• 作者：Bing Liang^a ; ¹ ; Jun Jie Deng^b ; ^c ; ¹ ; Fang Yuan^a ; Ning Yang^d ; Wei Li^a ; Jian Rui Yin^a ; Shu Xiang Pu^a ; Long Chang Xie^a ; Cong Gao^a ; ^{smilegaocong@126.com} ; Li Ming Zhang^c ; ^{ceszhlm@mail.sysu.edu.cn}
• 关键词：Star-shaped polymer ; ¦Â-Cyclodextrin ; Poly(amidoamine) dendron ; Gene delivery ; Neurotypic cells
• 刊名：Carbohydrate Polymers
• 出版年：2013
• 出版时间：15 April, 2013
• 年：2013
• 卷：94
• 期：1
• 页码：185-192
• 全文大小：1258 K

文摘

In order to develop the effective vectors that had high gene transfection capability and low cytotoxicity in the neuronal cells, we tested the star-shaped polymer consisting of ¦Â-cyclodextrin core and poly(amidoamine) (PAMAM) dendron arms [¦Â-CD-(D₃)₇] as the vector to transfect the human neuroblastoma SH-SY5Y cells. The physicochemical properties of the ¦Â-CD-(D₃)₇/plasmid DNA (pDNA) complexes were characterized by using gel electrophoresis, dynamic light scattering, transmission electron microscopy and zeta-potential experiments. Among the human neuroblastoma SH-SY5Y cells, ¦Â-CD-(D₃)₇/pDNA complex demonstrated a lower toxicity compared to those of PAMAM (G = 4, with an ethylenediamine core)/pDNA complex. When the N/P ratio was over 20, it was observed that PAMAM had a faster increment in toxicity compared to ¦Â-CD-(D₃)₇. Fluorescent image, confocal microscopy image and flow cytometry showed that ¦Â-CD-(D₃)₇/pDNA complexes had significantly higher transgene activity than that of PAMAM/pDNA complexes. For example, the transfection efficiency was 20 % and 7.5 % for ¦Â-CD-(D₃)₇/pDNA and PAMAM/pDNA complexes, respectively. These results indicated that ¦Â-CD-(D₃)₇ might be a promising candidate for neurotypic cells gene delivery with the characteristics of good biocompatibility, relatively high gene transfection capability and potential in vivo gene delivery ability.