Temporal changes in free iron levels after brain ischemia: Relevance to the timing of iron chelation therapy in stroke

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• 作者：Emilie Millerot-Serrurot ; Nathalie Bertr ; Claude Mossiat ; Philippe Faure ; Anne Prigent-Tessier ; Philippe Garnier ; Yannick Bejot ; Maurice Giroud ; Alain Beley ; Christine Marie
• 关键词：Free iron ; Ferritin ; Iron chelator ; Brain ischemia ; Rat
• 刊名：Neurochemistry International
• 出版年：2008
• 出版时间：June 2008
• 年：2008
• 卷：52
• 期：8
• 页码：1442-1448
• 全文大小：759 K

文摘

Whereas iron chelators have been proposed as therapeutic agents in stroke, changes in free iron levels have never been explored after focal brain ischemia. Therefore, free and total iron levels in cortical tissue and free iron levels in plasma were measured before and after (1, 4 and 24 h) photothrombotic occlusion of cortical vessels in rats. Brain ferritin expression and localization were also investigated before and after (24, 72 and 192 h) occlusion. The results showed that free iron remained below detectable levels in plasma and that the lesion exhibited high levels of free and total iron. As compared to contralateral values, free iron levels in ischemic core and penumbra increased (+50 % ) at 1 h and returned to control values at 4 h post-occlusion. In contrast, the increase in total iron levels (+20–30 % ) was long-lasting, but confined to the ischemic core. A time-dependent increase in the expression of both chains of ferritin was detected in regions that previously exhibited free iron accumulation. Finally, ischemic damage was reduced by the liposoluble iron chelator 2,2′-dipyridyl (20 mg/kg, i.p.) when injected 15 min or 1 h post-occlusion, yet not later (4 h).