A fluorophore-tagged RGD peptide to control endothelial cell adhesion to micropatterned surfaces

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• 作者：Corinne A. Hoesli ; Alain Garnier ; Pierre-Marc Juneau ; Pascale Chevallier ; Carl Duchesne ; Ga茅tan Laroche
• 关键词：Biomimetic material ; Cell spreading ; Endothelialisation ; Micropatterning ; Surface grafting ; Vascular grafts
• 刊名：Biomaterials
• 出版年：January, 2014
• 年：2014
• 卷：35
• 期：3
• 页码：879-890
• 全文大小：2089 K

文摘

The long-term patency rates of vascular grafts and stents are limited by the lack of surface endothelialisation of the implanted materials. We have previously reported that GRGDS and WQPPRARI peptide micropatterns increase the endothelialisation of prosthetic materials in聽vitro. To investigate the mechanisms by which the peptide micropatterns affect endothelial cell adhesion and proliferation, a TAMRA fluorophore-tagged RGD peptide was designed. Live cell imaging revealed that the micropatterned surfaces led to directional cell spreading dependent on the location of the RGD-TAMRA spots. Focal adhesions formed within 3聽h on the micropatterned surfaces near RGD-TAMRA spot edges, as expected for cell regions experiencing high tension. Similar levels of focal adhesion kinase phosphorylation were observed after 3聽h on the micropatterned surfaces and on surfaces treated with RGD-TAMRA alone, suggesting that partial RGD surface coverage is sufficient to elicit integrin signaling. Lastly, endothelial cell expansion was achieved in serum-free conditions on gelatin-coated, RGD-TAMRA treated or micropatterned surfaces. These results show that these peptide micropatterns mainly impacted cell adhesion kinetics rather than cell proliferation. This insight will be useful for the optimization of micropatterning strategies to improve vascular biomaterials.