Estimation of gastric pH in cynomolgus monkeys, rats, and dogs using [¹³C]-calcium carbonate breath test

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• 作者：Kazuki Tobita^a ; ^{tobitak@otsuka.jp" class="auth_mail" title="E-mail the corresponding author} ; Makoto Inada^a ; Asuka Sato^a ; Kimiyoshi Sudoh^a ; Hitoshi Sato^b
• 关键词：Breath test ; Ca13CO3 ; Gastric pH ; Proton pump inhibitor
• 刊名：Digestive and Liver Disease
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：48
• 期：9
• 页码：1035-1040
• 全文大小：502 K

文摘

The determination of gastric pH is important for the confirmation of efficacy of anti-secretory drugs. However, current methods for measurement of gastric pH provide significant stress to animals and humans.

Aim

The objective of this study is to establish an easy and reliable gastric pH measurement method by determining ¹³CO₂ concentration in expired air of monkeys, dogs, and rats after oral administration of Ca¹³CO₃.

Methods

A correlation of ¹³CO₂ concentration determined by a Ca¹³CO₃ breath test with gastric pH just before Ca¹³CO₃ administration was analyzed in the 3 animal species. The equations and contribution ratios of regression line were calculated from logarithmic ¹³CO₂ concentrations at 15 min after administration of Ca¹³CO₃ using the linear regression analysis.

Results

The ¹³CO₂ concentration in the Ca¹³CO₃ breath test was well correlated with the gastric pH just before Ca¹³CO₃ administration in the 3 animal species (r = −0.977 to −0.952). The equations of regression line between the ¹³CO₂ concentration and the gastric pH in each animal species showed good contribution ratios (R² ≥ 0.89).

Conclusions

The Ca¹³CO₃ breath test is an informative tool to estimate gastric pH in animals and will be applicable as a new noninvasive tool for patients with GERD/PPI-resistant symptoms.