Utilization of multiple substrates by butyrate kinase from Listeria monocytogenes

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• 作者：Sirisha Sirobhushanam ; Charitha Galva ; Lauren P. Saunders ; Suranjana Sen ; Radheshyam Jayaswal ; Brian J. Wilkinson ; Craig Gatto ; ^{cgatto@ilstu.edu}
• 刊名：Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：1862
• 期：3
• 页码：283-290
• 全文大小：790 K
• 卷排序：1862

文摘

Buk from L. monocytogenes exhibited broad substrate specificity. Buk preferred substrates with a chain length of 3 to 5 carbons. An alkyl side chain improved binding among shorter chain substrates. Buk could utilize unnatural substrates such as 2-ethylbutyrate, 2-methylpentanoate and 3-methylpentanoate. Buk catalysis involved ternary complex formation.