Chemo-profiling and bioassay of phytoextracts from Ageratum conyzoides for acaricidal properties against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) infesting cattle and buffaloes in India

详细信息    查看全文

• 作者：K.G. Ajith Kumar^a ; Amol B. Tayade^b ; Rajesh Kumar^b ; Suman Gupta^b ; Anil Kumar Sharma^a ; Gaurav Nagar^a ; Shashi Shankar Tewari^c ; Bhanu Kumar^c ; A.K.S. Rawat^c ; Sharad Srivastava^c ; Sachin Kumar^a ; Srikant Ghosh^a ; ^{sghoshtick@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Ageratum conyzoides ; Acaricide resistance ; Anti-tick activity ; Chemo-profiling ; GC/MS/MS ; Rhipicephalus (Boophilus) microplus
• 刊名：Ticks and Tick-borne Diseases
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：7
• 期：2
• 页码：342-349
• 全文大小：1015 K

文摘

In India, Rhipicephalus (Boophilus) microplus populations have developed a certain level of resistance to most of the acaricides marketed against tick species. To manage the problem, the present study was undertaken to evaluate the acaricidal potential of Ageratum conyzoides plants against acaricides-resistant ticks infesting cattle and buffaloes. The regression analysis of dose-response data of ethanolic extract of A. conyzoides revealed LC₉₀ value of 8.91% against reference susceptible IVRI-1 line of R.(B.) microplus. The ethanolic extract was found efficacious against 76.7–90% acaricides-resistant field ticks and adversely affected oviposition showing 7.04–31.3% reduction in egg laying capacity. The extract was also showed an in vitro efficacy of 52.5 and 76.7% against reference resistant IVRI-4 and 5 lines. The GC/MS/MS profiling of hexane extract, two bioactive sub-fractions and essential oils revealed the presence of 6,7-dimethoxy-2,2-dimethyl-2H-1-benzopyran (precocene II) as a major phyto-compound. The bioactive sub-fractions showed 96.2–97.5% efficacy against larvae of IVRI-1 and 77.1–94.9% against multi-acaricide resistant larvae of IVRI-5 line of R.(B.) microplus. The results of this study provided significant support for the development of a phyto-formulation based on A. conyzoides species.