Rapid detection and grouping of porcine bocaviruses by an EvaGreen® based multiplex real-time PCR assay using melting curve analysis
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- 作者：Xiaowen Zhenga ; 1 ; Gaopeng Liua ; 1 ; Tanja Opriessnigb ; c ; Zining Wanga ; Zongqi Yanga ; Yonghou Jianga ; yonghoujiang@zstu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：EvaGreen ; Multiplex real-time PCR ; Melting curve ; Porcine bocaviruses ; Genogroup ; Detection
- 刊名：Molecular and Cellular Probes
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：30
- 期：4
- 页码：195-204
- 全文大小：7386 K
文摘
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An EvaGreen®-based multiplex real-time PCR with melting curve analysis was developed for detection and grouping of PBoVs.
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The assay is specific, sensitive and cost-effective.
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This method could be an effective alternative for routine surveillance testing of multiple PBoVs in pigs.