Evaluation of highly sensitive immunoassay technologies for quantitative measurements of sub-pg/mL levels of cytokines in human serum
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- 作者：David Yeung1 ; Shawn Ciotti ; Shobha Purushothama1 ; Elham Gharakhani1 ; Geoffrey Kuesters1 ; Brian Schlain ; Chase Shen ; Douglas Donaldson ; Alvydas Mikulskis ; Alvydas.Mikulskis@biogen.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：FEAD ; frequency of endogenous analyte detection ; LBA ; ligand binding assay
- 刊名：Journal of Immunological Methods
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：437
- 期：Complete
- 页码：53-63
- 全文大小：621 K
文摘
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A comprehensive comparison of 9 immunoassay technologies and 4 cytokine assays
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Assay sensitivity for endogenous not recombinant protein that matters most
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Frequency of endogenous analyte detection (FEAD) is a useful measure of sensitivity.
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Correlation analysis in the absence of gold standard is critical to validate FEAD.
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Performance of IL-2, IL-17a, IL-6, and TNFα across multiple platforms summarized.