Oxidative damage: The biochemical mechanism of cellular injury and necrosis in choline deficiency

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• 作者：Marisa G. Repetto ; Georgina Ossani ; Alberto J. Monserrat ; Alberto Boveris
• 关键词：Lipid peroxidation ; Oxidative damage ; Choline deficiency ; Necrosis
• 刊名：Experimental and Molecular Pathology
• 出版年：2010
• 出版时间：February 2010
• 年：2010
• 卷：88
• 期：1
• 页码：143-149
• 全文大小：644 K

文摘

Oxidative stress and damage are characterized by decreased tissue antioxidant levels, consumption of tissue α-tocopherol, and increased lipid peroxidation. These processes occur earlier than necrosis in the liver, heart, kidney, and brain of weanling rats fed a choline deficient (CD) diet. In tissues, water-soluble antioxidants were analyzed as total reactive antioxidant potential (TRAP), α-tocopherol content was estimated from homogenate chemiluminescence (homogenate-CL), and lipid peroxidation was evaluated by thiobarbituric acid reactive substances (TBARS). Histopathology showed hepatic steatosis at days 1–7, tubular and glomerular necrosis in kidney at days 6 and 7, and inflammation and necrosis in heart at days 6 and 7. TRAP levels decreased by 18 % , 48 % , 56 % , and 66 % at day 7, with t_1/2 (times for half maximal change) of 2.0, 1.8, 2.5, and 3.0 days in liver, kidney, heart, and brain, respectively. Homogenate-CL increased by 97 % , 113 % , 18 % , and 297 % at day 7, with t_1/2 of 2.5, 2.6, 2.8, and 3.2 days in the four organs, respectively. TBARS contents increased by 98 % , 157 % , 104 % , and 347 % at day 7, with t_1/2 of 2.6, 2.8, 3.0, and 5.0 days in the four organs, respectively. Plasma showed a 33 % decrease in TRAP and a 5-fold increase in TBARS at day 5. Oxidative stress and damage are processes occurring earlier than necrosis in the kidney and heart. In case of steatosis prior to antioxidant consumption and increased lipid peroxidation, no necrosis is observed in the liver.