Precursor feeding studies and molecular characterization of geraniol synthase establish the limiting role of geraniol in monoterpene indole alkaloid biosynthesis in Catharanthus roseus leaves
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- 作者：Krishna Kumara ; b ; Sarma Rajeev Kumara ; b ; Varun Dwivedia ; b ; Avanish Raia ; b ; Ashutosh K. Shuklab ; Karuna Shankerc ; Dinesh A. Nagegowdaa ; b ; da.nagegowda@cimap.res.in" class="auth_mail" title="E-mail the corresponding author
- 关键词：EV ; empty vector ; G8O ; geraniol 10-hydroxylase/8-oxidase ; GES ; geraniol synthase ; GGPP ; geranylgeranyl diphosphate ; GPP ; geranyl diphosphate ; MeJA ; methyl jasmonate ; MEP ; methylerythritol phosphate ; MIA ; monoterpene indole alkaloids ; MPGR ; medicinal plant genomics resource database ; ORCA ; octadecanoid-responsive Catharanthus AP2/ERF domain ; qRT-PCR ; quantitative real-time PCR ; VIGS ; virus-induced gene silencing ; YFP ; yellow fluorescent protein
- 刊名：Plant Science
- 出版年：2015
- 出版时间：October 2015
- 年：2015
- 卷：239
- 期：Complete
- 页码：56-66
- 全文大小：1735 K
文摘
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Geraniol, but not tryptophan, feeding enhanced the MIA content in C. roseus leaves.
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CrGES expression is leaf and shoot specific and is induced by methyl jasmonate.
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CrGES-vigs reduced the MIA content, which was restored upon geraniol feeding.
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Overexpression of CrGES in C. roseus leaves increased the accumulation of MIAs.
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Thus, GES expression and geraniol availability play important role in MIA formation.