Derivation and characterization of the human embryonic stem cell line CR-4: Differentiation to human retinal pigment epithelial cells

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• 作者：John L. Mazzilli^a ; Aleksey Y. Domozhirov^a ; Stacey L. Mueller-Ortiz^a ; Charles A. Garcia^b ; Rick A. Wetsel^a ; ^{rick.a.wetsel@uth.tmc.edu} ; Eva M. Zsigmond^a
• 刊名：Stem Cell Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：18
• 期：Complete
• 页码：37-40
• 全文大小：724 K
• 卷排序：18

文摘

The CR-4 human embryonic stem cell line was derived from the inner cell mass of a developing blastocyst. This cell line has been adapted to grow in feeder-free conditions and is especially well-suited for differentiation to retinal pigment epithelium. The line demonstrates a normal human 46,XX female karyotype. Pluripotency was assessed through qRT-PCR for expression of NANOG, OCT-4, and SOX-2. A teratoma assay was performed and results were positive for all three germ layers. Testing for Mycoplasma was negative.