Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts

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• 作者：Marten Hansen^a ; Eszter Varga^a ; Tatjana Wü ; st^a ; Nelleke Brouwer^d ; Hugues Beauchemin^b ; Clemens Mellink^e ; Anne-Marie van der Kevie-Kersemaekers^e ; Tarik Mö ; rö ; y^b ; ^c ; Bert van der Reijden^d ; Marieke von Lindern^a ; Emile van den Akker^a ; ^{e.vandenakker@sanquin.nl}
• 刊名：Stem Cell Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：18
• 期：Complete
• 页码：26-28
• 全文大小：588 K
• 卷排序：18

文摘

Mobilized peripheral blood (MPB) CD34 + cells were cultured to CD41 +/CD34 + megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population that was reprogramed by a hOKSM self-silencing polycistronic vector using lentiviral delivery. The generated induced pluripotent stem cell (iPSC) lines were tested for silencing of the reprogramming construct by flow cytometry. Pluripotency of MML-6838-Cl2 iPSC line was confirmed by expression of associated markers and by in vivo spontaneous differentiation towards the 3 germ layers. The genomic integrity of iPSC line was shown by karyotyping. The MML-6838-Cl2 iPSC is, to our knowledge, the first to be generated from megakaryoblasts.