Generation of non-integrated induced pluripotent stem cells from a 23-year-old male with multiple endocrine neoplasia type 1 syndrome

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• 作者：Dongsheng Guo^a ; ^b ; ¹ ; Feima Wu^a ; ^b ; ¹ ; Haikun Liu^a ; ^b ; Ge Gao^a ; ^b ; Shanglong Kou^a ; ^b ; Fan Yang^a ; ^b ; Nasir Abbas^a ; ^b ; Tiancheng Zhou^b ; Xiujuan Cai^b ; Hui Zhang^b ; Dajiang Qin^b ; Jialiang Li^d ; Kecheng Xu^d ; ; Yin-xiong Li^a ; ^b ; ^c ; ^{li_yinxiong@gibh.ac.cn}
• 刊名：Stem Cell Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：18
• 期：Complete
• 页码：70-72
• 全文大小：537 K
• 卷排序：18

文摘

Urine resource cells were collected from a 23-year-old male with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cells with episomal plasmids. Two stable iPSC lines with free of episomal plasmid were established. The patient has a heterozygous G > T mutation on the exon 9 of Men1 gene that was confirmed by sequencing analysis on all resulted cell lines. Karyotyping indicated the chromosomes with normal appearances and numbers. Their pluripotency was demonstrated by gene expression and their abilities for differentiating into three germ layers. These iPSC lines provide valuable in vitro resources for pathological study on MEN1 syndrome.