Efficacy and toxicity of a tropically stable lipid-based formulation of amphotericin B (iCo-010) in a rat model of invasive candidiasis

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• 作者：Fady Ibrahim ; Pavel Gershkovich ; Olena Sivak ; Ellen K. Wasan ; Karen Bartlett ; Kishor M. Wasan
• 关键词：AmB ; amphotericin B ; CFU ; colony forming unit ; RES ; reticuloendothelial system ; VitE-TPGS ; d-alpha-tocopherol polyethylene glycol succinate ; HPLC ; high performance liquid chromatography ; QD ; every day ; TID ; three times a day
• 刊名：International Journal of Pharmaceutics
• 出版年：2012
• 出版时间：15 October, 2012
• 年：2012
• 卷：436
• 期：1-2
• 页码：318-323
• 全文大小：486 K

文摘

The objective of this work was to assess the antifungal activity of a tropically stable formulation of amphotericin B (AmB) (iCo-010) over short period of treatment in a rat model of invasive candidiasis. The rats were infected with Candida albicans (ATCC 18804); 48 h later, the animals were assigned either to a control group, AmBisome^? group (5 mg/kg QD), or iCo-010 groups (0.5, 1, 2.5, 5 and 10 mg/kg TID). The animals were treated for two days and then sacrificed 18 h following the completion of the treatment. The blood, liver, lungs, kidneys and spleen were harvested to assess the colony forming units in the samples. There was no significant difference in the reduction of the fungal burden in the organs between the AmBisome^? and iCo-010 groups except in the spleen and liver. There was a linear correlation between the antifungal activity in renal tissues and the administered doses of iCo-010. The plasma creatinine levels were not significantly different among the control and all the treatment groups. Oral iCo-010 has high efficacy against invasive candidiasis in renal and pulmonary tissues. Longer treatment period than the two-days regimen should be considered for higher therapeutic efficacy of iCo-010 in all the tissues.