Expression of CXCL4 and aquaporin 3 and 10 mRNAs in patients with otitis media with effusion

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• 作者：Zhe Jin^a ; Sung Ho Cha^b ; Yong-Sung Choi^b ; Young Il Kim^c ; Sun A. Choi^d ; Seung Geun Yeo^c ; ^d ; ^{yeo2park@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Otitis media with effusion (OME) ; CXCL4 and 7 ; Aquaporin 3 and 10
• 刊名：International Journal of Pediatric Otorhinolaryngology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：81
• 期：Complete
• 页码：33-37
• 全文大小：275 K

文摘

Bacterial infections in children with underdeveloped Eustachian tubes are a major cause of otitis media with effusion (OEM), and persistent effusion in the middle ear in these patients is a major cause of surgical intervention. CXCL4 is associated with bacterial infection, and aquaporins 3 and 10 are associated with water metabolism. This study assessed the expression of mRNAs encoding CXCL-4 and aquaporins 3 and 10 in the effusion of pediatric OME patients, and the association of this expression with clinical manifestations.

Methods

Levels of CXCL4 and aquaporin 3 and 10 mRNA were assayed by real-time RT-PCR in the middle ear effusion of 38 pediatric patients with OME requiring ventilation tube insertion. The relationships of these mRNA levels with the presence of bacteria; concomitant diseases such as allergic rhinitis, sinusitis, and adenoid disease; recurrence of OME; and number of ventilation tube insertions were evaluated.

Results

CXCL4 and aquaporin 3 and 10 mRNAs were expressed in middle ear effusion of all OME patients. CXCL-4 mRNA levels were significantly lower when bacteria were present and in patients with concomitant diseases (p < 0.05 each). Levels of all three mRNAs were unrelated to OME recurrence or number of ventilation tube insertions (p > 0.05 each). The levels of CXCL4 and aquaporin 10 mRNAs were significantly correlated (p < 0.05).

Conclusion

Expression of CXCL4 and aquaporin 3 and 10 mRNAs in middle ear effusion is associated with the pathophysiology of OME. CXCL4 mRNA levels are significantly lower in patients with than without concomitant diseases or bacterial infections.