Additive effects of TEGDMA and hydrogenperoxide on the cellular glutathione content of human gingival fibroblasts

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• 作者：Joachim Volk ; Gabriele Leyhausen ; Sami Dogan ; Werner Geurtsen
• 关键词：Gingival fibroblasts ; TEGDMA ; H₂O₂ ; Glutathione
• 刊名：Dental Materials
• 出版年：2007
• 出版时间：August 2007
• 年：2007
• 卷：23
• 期：8
• 页码：921-926
• 全文大小：303 K

文摘

Objectives

Only few data are available about cytotoxic effects of leachable dental resin compounds in combination with hydrogen peroxide (H₂O₂) segregated from dental bleaching agents. Therefore, the purpose of this study was to evaluate the effects of various concentrations of triethylene-glycol dimethacrylate (TEGDMA) and H₂O₂ on intracellular glutathione levels (GSH) and viability of human gingival fibroblasts (HGF) that are primary target cells of cytotoxic actions of these substances.

Methods

HGF were grown in 96-well plates for 24 h, treated with various concentrations of TEGDMA (0.5–5.0 mM) for 24 h and subsequently for 90 min with 0.2 mM H₂O₂ or culture medium (control). The relative intracellular GSH concentration was determined using a fluorescence assay with monobromobimane. Readings were normalized to cell numbers, which were determined by a propidium iodide assay. Data were statistically analyzed by t-test and ANOVA with Tukey's post test. A significance level of p < 0.05 was used.

Results

Exposure to TEGDMA reduced the viability of HGF at concentrations ≥1.0 mM. TEGDMA induced a decrease of the GSH pool in a concentration-dependent manner (p < 0.05). The depletion of GSH was correlated with a reduction of viability (p < 0.05) and the total cell number. Furthermore, a significant decrease of the intracellular GSH content was found when cells were exposed to TEGDMA in combination with H₂O₂, compared to experiments without H₂O₂.

Significance

We conclude from our findings that TEGDMA and H₂O₂ have additive adverse effects on GSH metabolism and cell viability.