Cytotoxicity and induction of DNA double-strand breaks by components leached from dental composites in primary human gingival fibroblasts

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• 作者：Mohamed Shehata ; J¨¹rgen Durner ; Ayce Eldenez ; Kirsten Van L ; uyt ; Panorea Styllou ; Lena Rothmund ; Reinhard Hickel ; Harry Scherthan ; Werner Geurtsen ; Bernd Kaina ; Thomas Carell ; Franz X. Reichl
• 关键词：Composite ; TEEGMA ; TPP ; TPSB ; Neopen ; DPIC ; ¦Ã-H2AX assay ; HGF
• 刊名：Dental Materials
• 出版年：2013
• 出版时间：September, 2013
• 年：2013
• 卷：29
• 期：9
• 页码：971-979
• 全文大小：1256 K

文摘

Introduction

The public interest steadily increases in the biological adverse effects caused by components released from resin-based dental restorations.

Objective

In this study, the cytotoxicity and the genotoxicity were investigated of following released components from dental resin restorations in human gingival fibroblasts (HGF): tetraethyleneglycol dimethacrylate (TEEGDMA), neopentylglycol dimethacrylate (Neopen), diphenyliodoniumchloride (DPIC), triphenyl-stibane (TPSB) and triphenylphosphane (TPP).

Methods

XTT based cell viability assay was used for cytotoxicity screening of substances. ¦Ã-H2AX assay was used for genotoxicity screening. In the ¦Ã-H2AX assay, HGFs were exposed to the substances for 6 h. Induced foci represent double DNA strand breaks (DSBs), which can induce ATM-dependent phosphorylation of the histone H₂AX. Cell death effects (apoptosis and necrosis), induced by the substances were visually tested by the same investigator using the fluorescent microscope.

Results

All tested substances induced a dose-dependent loss of viability in HGFs. Following toxicity ranking among the substances at EC₅₀-concentration were found in the XTT assay (mM, mean ¡À SEM; n = 5): DPIC > Neopen > TPSB > TPP > TEEGDMA.

DSB-foci per HGF-cell were obtained, when HGFs were exposed to the EC₅₀-concentration of each substance in the following order (mean ¡À SEM; n = 3): DPIC > Neopen > TPSB > TPP > TEEGDMA.

Multi-foci cells (cells that contain more than 40 foci each) in 80 HGF-cells at EC₅₀-concentration of each substance were found as follow (mean ¡À SEM; n = 3): DPIC > Neopen > TPP > TPSB > TEEGDMA.

Cell apoptosis contained in each substance at EC₅₀-concentration in the following order (mean ¡À SEM; n = 3): DPIC > Neopen > TPSB > TPP >TEEGDMA. Cell necrosis contained in each substance at EC₅₀-concentration in the following order (mean ¡À SEM; n = 3): DPIC > Neopen > TPSB > TPP > TEEGDMA.

Conclusion

Leached components from dental resin restorations can induce DNA DSBs and cell death effects in HGFs.