Severity-dependent expression of pro-inflammatory cytokines in traumatic spinal cord injury in the rat

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• 作者：Liqun Yang ; Nigel R. Jones ; Peter C. Blumbergs ; Corinna Van Den Heuvel ; Emma J. Moore ; Jim Manavis ; Ghafar T. Sarvestani ; Mounir N. Ghabriel
• 关键词：interleukin-1β ; interleukin-6 ; tumour necrosis factor-α ; traumatic spinal cord injury ; microglia ; inflammatory response
• 刊名：Journal of Clinical Neuroscience
• 出版年：2005
• 出版时间：April 2005
• 年：2005
• 卷：12
• 期：3
• 页码：276-284
• 全文大小：521 K

文摘

The post-traumatic inflammatory response in acute spinal cord contusion injury was studied in the rat. Mild and severe spinal cord injury (SCI) was produced by dropping a 10 g weight from 3 and 12 cm at the T12 vertebral level. Increased immunoreactivity of TNF-α in mild and severe SCI was detected in neurons at 1 h post-injury, and in neurons and microglia at 6 h post-injury, with a less significant increase in mild SCI. Expression was short-lived and declined sharply by 1 d post-injury. RT-PCR showed an early significant up-regulation of IL-1β, IL-6 and TNF-α mRNAs, maximal at 6 h post-injury with return to control levels by 24 h post-injury, the changes being less statistically significantly in mild SCI. Western blot showed early transient increases of IL-1β, IL-6 and TNF-α proteins in severe SCI but not mild SCI. Immunocytochemical, western blotting and RT-PCR analyses suggest that endogenous cells (neurons and microglia) in the spinal cord, not blood-borne leucocytes, contribute to IL-1β, IL-6 and TNF-α production in the post-traumatic inflammatory response and that their up-regulation is greater in severe than mild SCI.