Evaluation of mutagenic and antimutagenic activities of oligorutin and oligoesculin
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- 作者：Ghada Ben Rhouma ; Latifa Chebil ; Mounira Krifa ; Mohamed Ghoul ; Leila Chekir-Ghedira
- 关键词：Enzymatic polymerisation ; Rutin ; Esculin ; Trametes versicolor ; Ames assay
- 刊名：Food Chemistry
- 出版年：2012
- 出版时间：1 December, 2012
- 年：2012
- 卷：135
- 期：3
- 页码：1700-1707
- 全文大小：359 K
文摘
Rutin and esculin have been polymerised by laccase. Five fractions with between 2127.42 and 8331.85 g/mol for oligorutins, and between 688.12 and 6973 g/mol for oligoesculins, were obtained. Fourier transformed infrared analysis showed that oligorutins were formed through C-C, C-O and CO linkages, while oligoesculins were obtained through C-C linkages.
Monomers, their oligomers and their metabolites exhibited no mutagenic effect. Oligorutins and oligoesculins were more efficient in reducing the mutagenicity of methyl methanesulphonate, by, respectively, 69 % and 64.8 % in the presence of Salmonella typhimurium TA104, and 79.7 % and 68.9 % in the presence of S. typhimurium TA102, than were their monomers. The same oligomers revealed greater significant inhibitory effect of 2-aminoanthracene mutagenicity (respectively 82.4 % and 79.3 % in the presence of S. typhimurium TA104, and 89.2 % and 82.9 % in the presence of S. typhimurium TA102), than their monomers. Our results strongly suggest the enhancement of the tested monomer antimutagenicity after polymerisation.