In vitro fluconazole susceptibility of 1565 clinical isolates of candida species evaluated by the disk diffusion method performed using NCCLS M44-A guidelines

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• 作者：Testore ; Gian Piero ; Dori ; Luca ; Buonomini ; Anna Rita ; Schito ; Gian Carlo ; Soro ; Ornella ; Fortina ; Giacomo ; et. al.
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2004
• 出版时间：November, 2004
• 年：2004
• 卷：50
• 期：3
• 页码：187-192
• 全文大小：104 K

文摘

We determined the in vitro activity of fluconazole against 1565 clinical Candida spp. isolates collected from different specimens of non-AIDS outpatients and inpatients in 3 different regions of Italy. Susceptibility testing was performed by agar disk diffusion using the NCCLS document M44-A guidelines. Candida albicans was the most frequently isolated yeast (68 % ) followed by C. glabrata (15 % ), C. tropicalis (5 % ), C. parapsilosis (5 % ), and C. krusei (5 % ). Other yeasts represented 4 % of all isolates. Of the 1565 isolates tested, 1449 (92.6 % ) were susceptible (S) to fluconazole, 43 (2.7 % ) were susceptible dose-dependent (S-DD) and 73 (4.7 % ) were resistant (R). Almost all (98.2 % ) of the C. albicans isolates were classified as S or S-DD. Despite its widespread use, fluconazole displayed good activity against the isolates we tested, and the disk diffusion method was confirmed as a reliable approach to the evaluation of in vitro susceptibility of yeasts to this antimycotic agent.