Germ cell apoptosis control during spermatogenesis

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• 作者：Claudia Giampietri ; Simonetta Petrungaro ; Pierpaolo Coluccia ; Alessio D'Alessio ; Donatella Starace ; Anna Riccioli ; Fabrizio Padula ; Fioretta Palombi ; Elio Ziparo ; Antonio Filippini and Paola De Cesari
• 关键词：c-Flip ; Testis ; Spermatocytes ; Spermatogonia ; Caspases
• 刊名：Contraception
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：72
• 期：4
• 页码：298-302
• 全文大小：287 K

文摘

The aim of the present study was to investigate the expression and role of c-Flip long isoform (c-Flip_L), a known anti-apoptotic protein. No data are currently available on c-Flip_L in male gonad before puberty; therefore, this study was carried out in prepuberal mouse testis. We investigated pachytene spermatocytes and spermatogonia by immunostaining of testis sections and found a strong and specific expression of c-Flip_L in pachytene spermatocytes, while spermatogonia expressed very low levels of c-Flip_L. This finding inversely correlated with the caspases activity, which was higher in spermatogonia as compared to pachytene spermatocytes. Other experiments carried out in an organ-culture model revealed that Fas-induced apoptosis was higher in spermatogonia as compared to pachytene spermatocytes. These data suggest that c-Flip_L may play a role as an anti-apoptotic molecule in the prepuberal mouse testis and open new perspectives in the comprehension of the mechanisms controlling germ cells apoptosis.