FT-IR study of heterologous protein expression in recombinant Escherichia coli strains

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• 作者：Ami ; Diletta ; Bonecchi ; Loredana ; Calì ; Simona ; Orsini ; Gaetano ; Tonon ; Giancarlo ; Doglia ; Silvia Maria
• 关键词：Inclusion body ; FT-IR microspectroscopy ; Escherichia coli ; Expression of heterologous protein ; Interferon alpha
• 刊名：Biochimica et Biophysica Acta (BBA)/General Subjects
• 出版年：2003
• 出版时间：December 5, 2003
• 年：2003
• 卷：1624
• 期：1-3
• 页码：6-10
• 全文大小：321 K

文摘

Two recombinant Escherichia coli strains expressing different levels of an interferon fusion protein as inclusion bodies have been studied by Fourier transform infrared (FT-IR) microspectroscopy. A marker band at 1628 cm⁻¹ allowed monitoring of the protein expression by direct analysis of cell pellets in a rapid, non-invasive and quantitative way. The results demonstrate that FT-IR microspectroscopy is a technique of potential biotechnological interest for studying inclusion body formation.