The influence of cumulus cells during in vitro fertilization of buffalo (Bubalus bubalis) denuded oocytes that have undergone vitrification

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• 作者：Laura Attanasio ; Anna De Rosa ; Marina De Blasi ; Gianluca Neglia ; Luigi Zicarelli ; Giuseppe Campanile ; Bianca Gasparrini
• 关键词：Buffalo ; Oocyte vitrification ; Cumulus cells
• 刊名：Theriogenology
• 出版年：2010
• 出版时间：November 2010
• 年：2010
• 卷：74
• 期：8
• 页码：1504-1508
• 全文大小：305 K

文摘

The aim of this work was to evaluate whether providing a support of cumulus cells during IVF of buffalo denuded oocytes submitted to vitrification-warming enhances their fertilizing ability. In vitro matured denuded oocytes were vitrified by Cryotop in 20 % EG + 20 % of DMSO and 0.5 M sucrose and warmed into decreasing concentrations of sucrose (1.25 M–0.3M). Oocytes that survived vitrification were fertilized: 1) in the absence of a somatic support (DOs); 2) in the presence of bovine cumulus cells in suspension (DOs+susp); 3) on a bovine cumulus monolayer (DOs+monol); and 4) with intact bovine COCs in a 1:1 ratio (DOs+COCs). In vitro matured oocytes were fertilized and cultured to the blastocyst stage as a control.

An increased cleavage rate was obtained from DOs+COCs (60.9 % ) compared to DOs, DOs+susp (43.6 and 38.4, respectively; P < 0.01) and DOs+monol (47.5 % ; P < 0.05). Interestingly, cleavage rate of DOs+COCs was similar to that of fresh control oocytes (67.8 % ). However, development to blastocysts significantly decreased in all vitrification groups compared to the control (P < 0.01).

In conclusion the co-culture with intact COCs during IVF completely restores fertilizing capability of buffalo denuded vitrified oocytes, without improving blastocyst development.