Enhancement on the Europium emission band of Europium chlortetracycline complex in the presence of LDL
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- 作者：Luciane dos Santos Teixeira ; André ; a Nastri Grasso ; Andrea Moreira Monteiro ; A.M. Figueiredo Neto ; Nilson Dias Vieira Jr. ; Magnus Gidlund ; Lilia Coronato Courrol
- 关键词：LDL ; Europium ; Fluorescence ; Biosensor
- 刊名：Analytical Biochemistry
- 出版年：2010
- 出版时间：1 May 2010
- 年：2010
- 卷：400
- 期：1
- 页码：19-24
- 全文大小：1122 K
文摘
Low-density lipoprotein (LDL) particles are the major cholesterol-carrying lipoprotein in the human circulation from the liver to peripheral tissues. High levels of LDL-Cholesterol (LDL-C) are known risk factor for the development of coronary artery disease (CAD). The most common approach to determine the LDL-C in the clinical laboratory involves the Friedewald formula. However, in certain situations, this approach is inadequate. In this paper we report on the enhancement on the Europium emission band of Europium chlortetracycline complex (CTEu) in the presence of LDL. The emission intensity at 615 nm of the CTEu increases with increasing amounts of LDL. This phenomenon allowed us to propose a method to determine the LDL concentration in a sample composed by an aqueous solution of LDL. With this result we obtained LDL calibration curve, LOD (limit of detection) of 0.49 mg/mL and SD (standard deviation) of 0.003. We observed that CTEu complex provides a wider dynamic concentration-range for LDL determination than that from Eu–tetracycline previously. The averaged emission lifetimes of the CTEu and CTEu with LDL (1.5 mg/mL) complexes were measured as 15 and 46 μs, respectively. Study with some metallic interferents is presented.