Large-scale time-lapse microscopy of Oct4 expression in human embryonic stem cell colonies
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- 作者：Kiran Bhadrirajua ; Michael Halterb ; ; Julien Amelotc ; Peter Bajcsyc ; Joe Chalfounc ; Antoine Vandecremec ; Barbara S. Mallond ; Kye-yoon Parkd ; Subhash Sistab ; John T. Elliottb ; Anne L. Plantb
- 关键词：hESC ; human embryonic stem cell ; EGFP ; enhanced green fluorescent protein ; FOV ; field of view ; TB ; terabyte ; GB ; gigabyte
- 刊名：Stem Cell Research
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：17
- 期：1
- 页码：122-129
- 全文大小：1342 K
文摘
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Quantitative characterization of growth rate and other parameters over hundreds of stem cell colonies
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Tracking of Oct4 expression through green fluorescent protein over hundreds of colonies at close to cell resolution
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Identification of rare events that would be easily missed by visual inspection of a very large data set