Cellular prion protein regulates the motor behaviour performance and anxiety-induced responses in genetically modified mice

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• 作者：Bruno Lobã ; o-Soares ; Roger Walz ; Carlos Gilberto Carlotti Jr. ; Amé ; rico Ceiki Sakamoto ; Fabrd ; cio Calvo ; Ana Luiza Bernardes Terzian ; Juliana Almeida da Silva ; Lauro Wichert-Ana ; Norberto
• 关键词：Cellular prion protein ; Motor behaviour ; Plus-maze test ; Open field test ; Rota-rod test ; Anxiety
• 刊名：Behavioural Brain Research
• 出版年：2007
• 出版时间：1 October 2007
• 年：2007
• 卷：183
• 期：1
• 页码：87-94
• 全文大小：423 K

文摘

The cellular prion protein (PrP^C) is a sialoglycoprotein involved in neuroplasticity processes and synaptic transmission. This study investigated behavioural responses (balance in the rota-rod test at 24 rpm, motility in the open-field test, anxiety in the elevated plus-maze test) in Zurich developed wild-type adult mice (WT, controls of normal PrP^C expression), in knockout (KO) mice (Prnp^0/0, with no PrP^C expression), and in PrP^C overexpressing Tg-20 mice. After 8 min in the rota-rod test, Tg-20 animals presented significantly fewer falls (1.08 ± 1.56 falls) than both WT (7.27 ± 4.36) and KO (7.6 ± 6.15) mice (p < 0.01). In the open field test, Tg-20 animals showed significantly increased motility [rearing = 23.4 ± 7.85, crossing = 97.30 ± 32.11) when compared with KO mice (rearing = 5.45 ± 3.69 and crossing = 59.73 ± 15.43) or WT mice (rearing = 6.5 ± 20.23 and crossing = 45.18 ± 20.33) (p < 0.01). In the elevated plus-maze test, Tg-20 mice showed less anxiety (head projections = 7.3 ± 1.62) when compared with WT animals (3.38 ± 0.67) (p < 0.05). Moreover, KO mice spent more time in the centre of the plus maze (37.80 ± 5.57 s) than did WT mice (22.57 ± 3.82) (p < 0.05). PrP^C overexpressing mice evoked increased motility, less anxiety, and increased equilibrium when compared with WT control animals in the behavioural protocols used. KO animals also tended to evoke fewer anxiety-related responses in the elevated plus-maze test. These findings indicate that the levels of PrP^C in adult life are associated with possible changes in motility, anxiety, and equilibrium.