Colonized beads as inoculum for marine biodegradability assessment: Application to Linear Alkylbenzene Sulfonate

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• 作者：Aourell Mauffret ; André ; Rottiers ; Thomas Federle ; David C. Gillan ; Miriam Hampel ; Julian Blasco ; Ali Temara
• 关键词：Marine ; Biodegradation ; LAS ; Colonized beads ; Biofilm
• 刊名：Environment International
• 出版年：2009
• 出版时间：August 2009
• 年：2009
• 卷：35
• 期：6
• 页码：885-892
• 全文大小：775 K

文摘

An innovative biodegradation test system was developed in order to fill the current gap for cost effective and environmentally relevant tools to assess marine biodegradability. Glass beads were colonized by a biofilm in an open flow-through system of seawater with continuous pre-exposure to Linear Alkylbenzene Sulfonate (LAS) (20 ;c;g/L). Thereafter, such colonized beads were added as inoculum in different test systems. [¹⁴C]-LAS (5–100 ;c;g/L) was added and primary and ultimate biodegradation were assessed. The bacterial density collected on the beads (10⁹ bact./mL beads) was ca. 3 orders of magnitude higher than the typical seawater content. The LAS mineralization lag phase duration decreased from 55 to c; 1 days and the mineralization extent increased from 53 to 90 % as the colonized beads volume increased from 10 to 275 mL. This is the first demonstration of marine bacteria's ability to mineralize LAS. On the opposite, less than 13 % LAS was mineralized in seawater only. The colonized beads possibly enhanced the probability to encounter the full degraders' consortium in a low volume of seawater (100 mL).