This study investigated the setting ti
me and
micohardness of a pre
mixed calciu
m phosphate silicate–based sealer (EndoSequence BC Sealer; Brasseler USA, Savannah, GA) in the presence of different
moisture contents (0–9 wt%). The
moisture content that produced the
most opti
mal setting properties was used to prepare set EndoSequence BC Sealer for cytotoxicity co
mparison with an epoxy resin–based sealer (AH Plus; Dentsply Caulk, Milford, DE).
Methods
Standardized disks were created with BC Sealer, AH Plus, Pulp Canal Sealer EWT (positive control) (SybronEndo, Orange CA), and Teflon (Small Parts Inc., Miami Lakes, FL; negative control). Disks were placed in Transwell Inserts, providing indirect contact with MC3T3-E1 cells. Succinate dehydrogenase activity of the cells was evaluated over a 6-week period using MTT ((3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Cytotoxicity profiles of BC Sealer and AH Plus were fitted with polynomial regression models. The time for 50% of the cells to survive (m>T0.5m>) was analyzed using the Wald statistic with a two-tailed significance level of 0.05.
Results
BC Sealer required at least 168 hours to reach the final setting using the Gilmore needle method, and its microhardeness significantly declined when water was included in the sealer (m>Pm> = .004). All set sealers exhibited severe cytotoxicity at 24 hours. The cytotoxicity of AH Plus gradually decreased and became noncytotoxic, whereas BC Sealer remained moderately cytotoxic over the 6-week period. A significant difference (m>Pm> < .001) was detected between m>T0.5m> of BC Sealer (5.10 weeks; 95% confidence interval [CI], 4.69–5.42, standard error [SE] = 0.09) and m>T0.5m> of AH Plus (0.86 weeks; 95% CI, 0.68–1.05; SE = 0.18).
Conclusions
Further studies are required to evaluate the correlation between the length of setting time of BC Sealer and its degree of cytotoxicity.