A complete inventory of fungal kinesins in representative filamentous ascomycetes

详细信息    查看全文

• 作者：Schoch ; Conrad L. ; Aist ; James R. ; Yoder ; Olen C. ; Gillian Turgeon ; B.
• 关键词：Kinesin ; Motor proteins ; Protein families ; Fungi ; Ascomycetes ; Phylogenomics ; Phylogenetics
• 刊名：Fungal Genetics and Biology
• 出版年：2003
• 出版时间：June, 2003
• 年：2003
• 卷：39
• 期：1
• 页码：1-15
• 全文大小：700 K

文摘

Complete inventories of kinesins from three pathogenic filamentous ascomycetes, Botryotinia fuckeliana, Cochliobolus heterostrophus, and Gibberella moniliformis, are described. These protein sequences were compared with those of the filamentous saprophyte, Neurospora crassa and the two yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. Data mining and phylogenetic analysis of the motor domain yielded a constant set of 10 kinesins in the filamentous fungal species, compared with a smaller set in S. cerevisiae and S. pombe. The filamentous fungal kinesins fell into nine subfamilies when compared with well-characterized kinesins from other eukaryotes. A few putative kinesins (one in B. fuckeliana and two in C. heterostrophus) could not be defined as functional, due to unorthodox organization and lack of experimental data. The broad representation of filamentous fungal kinesins across most of the known subfamilies and the ease of gene manipulation make fungi ideal models for functional and evolutionary investigation of these proteins.