Detection and quantification of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Streptococcus oralis in blood samples with different microbiological identification methods: An in vitro study

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• 作者：Marí ; a José ; Marinp>ap> ; Nagore Ambrosiop>ap> ; Leire Virtop>ap> ; Pedro Dizp>bp> ; Maximiliano Á ; lvarezp>cp> ; David Herrerap>dp> ; Mariano Sanzp>dp> ; Elena Figuerop>ap>p> ; p>p>dp>p> ; p>p>elfiguer@ucm.esp>
• 关键词：Bacteremia ; Quantitative PCR ; Culture ; Periodontal ; Aggregatibacter actinomycetemitans ; Porphyromonas gingivalis
• 刊名：Archives of Oral Biology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：74
• 期：Complete
• 页码：55-62
• 全文大小：491 K
• 卷排序：74

文摘

Direct anaerobic culture (DAC) reported the best results. DAC, lysis-centrifugation and qPCR were able to detect all concentrations. A detection limit for qPCR at 102 CFU/mL for P. gingivalis is advisable. A detection limit for qPCR at 104 CFU/mL for S. oralis is advisable.