Chemiluminescence imaging in bioanalysis
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- 作者：Pasini ; Patrizia ; Musiani ; Monica ; Russo ; Carmela ; Valenti ; Piero ; Aicardi ; Giorgio ; E. Crabtree ; Jean ; et. al.
- 关键词：Biospecific probes ; Chemiluminescence imaging ; Chemiluminescence coupled enzymatic reactions ; Chemiluminescence immunohistochemistry ; Chemiluminescence in situ hybridization ; High throughput screening
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：1998
- 出版时间：December 1, 1998
- 年：1998
- 卷：18
- 期：4-5
- 页码：555-564
- 全文大小：1.20 M
文摘
The development, analytical performance and applications of chemiluminescence imaging as a tool for quantitative analyte localization in target biological specimens are described. The detection of acetylcholinesterase activity both in array format and on a target surface are described. A proposed application of the method is a 384 well microtiter format assay for high throughput screening of acetylcholinesterase inhibitors such as tacrine, a drug widely used in the treatment of Alzheimer’s disease, and two recently developed analogues. The chemiluminescent system in conjunction with optical microscopy allowed localization of acetylcholinesterase in brain tissue sections. We also describe the chemiluminescent immunohistochemical localization of interleukin 8 in Helicobacter pylori infected gastric mucosa cryosections and an in situ hybridization assay for the detection of herpes simplex virus DNA in single cells.