Inactivation of mushroom polyphenoloxidase in model systems exposed to high-pressure carbon dioxide
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- 作者：Lara Manzoccoa ; lara.manzocco@uniud.it" class="auth_mail" title="E-mail the corresponding author ; Alexandra Ignata ; Fabio Valoppia ; Krystel Rita Burrafatoa ; Giovanna Lippea ; Sara Spilimbergob ; Maria Cristina Nicolia
- 关键词：Dense-phase CO2 ; Tyrosinase ; Inactivation kinetics ; SDS&ndash ; PAGE
- 刊名：The Journal of Supercritical Fluids
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：107
- 期：Complete
- 页码：669-675
- 全文大小：883 K
文摘
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HP-CO2 treatments quickly inactivated mushroom polyphenoloxidase in model systems.
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Pressure sensitivity (zP) of enzyme inactivation was circa 5.5 MPa at 20 and 35 °C.
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At 20 and 35 °C, activation volume ΔV≠ was about −1000 cm3 mol−1.
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HP-CO2 did not change the molecular mass of the enzyme subunits.