Urine sediment analysis: Analytical and diagnostic performance of sediMAX^® — A new automated microscopy image-based urine sediment analyser

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• 作者：Zahur Zaman ; Giovanni Battista Fogazzi ; Giuseppe Garigali ; Maria Daniela Croci ; Gabor Bayer ; Tunde Krá ; nicz
• 关键词：Urine sediment analysis ; Automated urine analysis ; Urine microscopy ; Urine particles ; sediMAX/Urised
• 刊名：Clinica Chimica Acta
• 出版年：2010
• 出版时间：2 February 2010
• 年：2010
• 卷：411
• 期：3-4
• 页码：147-154
• 全文大小：429 K

文摘

Background

We evaluated the analytical and diagnostic performance of sediMAX (77 Elektronika, Budapest, Hungary), a new automated microscopy image-based urine sediment analyser (which in some countries is also called Urised) in comparison with visual phase-contrast microscopy.

Methods

Precision, linearity, carry-over and method comparison were carried out according to well-established guidelines. The diagnostic performance with respect to visual phase-contrast microscopy was evaluated with results from two centres (nb>1b> = 910, nb>2b> = 1233). Uncentrifuged urine samples were used in visual microscopy in centre 1, while urine sediments were used in centre 2.

Results

Within-run precision for RBC was 17.8 % and 6.7 % at 18 × E6 RBC/L and 447 × E6 RBC/L respectively and for WBC it was 17 % and 4.4 % at 4 × E6 WBC/L and 258 × E6/L respectively. Between-run imprecision for RBC was 14.7 % for 30 × E6/L and 7.2 % for 283 × E6/L. For WBC it was 5.4 % at 25 × E6/L and 3 % at 166 × E6/L. In both studies areas under ROC curves (AUC) were 80–90 % for RBC, WBC, squamous epithelial cells, yeast and calcium-oxalate crystals. For non-squamous epithelial cells and pathological and hyaline casts the AUC ranged 73–74 % . There was no carry-over.

Conclusions

The sediMAX is well able to count and identify RBC, WBC, squamous epithelial cells, yeast, bacteria and calcium-oxalate crystals. Recognition of pathological casts and non-squamous epithelial cells is adequate but needs to be improved.