High spontaneous granulocyte/macrophage-colony formation in patients with myelofibrosis

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• 作者：Verena Hauer ; Eva J盲ger ; Thamer Sliwa ; Ralph Simanek ; Heinz Gisslinger ; Ulrich J盲ger ; Klaus Geissler
• 关键词：Myelofibrosis ; CFU-GM ; Spontaneous ; Cultures ; Methylcellulose
• 刊名：Leukemia Research
• 出版年：January, 2014
• 年：2014
• 卷：38
• 期：1
• 页码：116-120
• 全文大小：572 K

文摘

Unstimulated methylcellulose cultures in 25 myelofibrosis (MF) patients were performed to better understand the role of cytokines in the proliferation of MF cells. Compared to controls MF patients show a variable but highly increased spontaneous CFU-GM formation (66 vs 4.8/10⁵ PBMNC). There was a marked reduction of autonomous CFU-GM growth by the cytokine-synthesis-inhibiting molecule IL-10 as well as by antibodies against GM-CSF whereas antibodies against IL-3, G-CSF, M-CSF and IL-1尾 showed heterogeneous effects. Spontaneous CFU-GM growth >100/10⁵ PBMNC predicted shorter survival. Constitutive release of GM-CSF seems to contribute to proliferation of MF cells in vitro and possibly in vivo.