Fasciola hepatica: Comparison of the sedimentation and FLOTAC techniques for the detection and quantification of faecal egg counts in rats

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• 作者：Urs Duthaler ; Laura Rinaldi ; Maria P. Maurelli ; Mireille Vargas ; Jü ; rg Utzinger ; Giuseppe Cringoli ; Jennifer Keiser
• 关键词：Fasciola hepatica ; Diagnosis ; Sedimentation ; FLOTAC ; Sensitivity ; Faecal egg count ; Efficiency
• 刊名：Experimental Parasitology
• 出版年：2010
• 出版时间：October 2010
• 年：2010
• 卷：126
• 期：2
• 页码：161-166
• 全文大小：242 K

文摘

We compared the sedimentation and FLOTAC techniques for the detection and quantification of Fasciola hepatica eggs in faecal samples obtained from 120 experimentally-infected rats before intervention, and in 42 rats after drug administration. Additionally, the average time for a single test was determined. A single FLOTAC showed a higher sensitivity (92.6 % ) than 2, 4 and 8 sedimentation readings (63.0–85.2 % ) for detecting F. hepatica eggs in rat faeces post-treatment. On average, it took 21 min to prepare and examine a single FLOTAC, whereas 114 min were needed for the sedimentation method including the reading of 8 slides. In both treated and untreated rats, the sedimentation method resulted in higher mean faecal egg counts (FECs) than FLOTAC (P < 0.05). In view of the high sensitivity and efficiency, the FLOTAC technique holds promise for experimental work in the F. hepatica-rat model. Additional research is needed to determine the reasons for the observed differences in FECs.