文摘
Mass isotope labeling combined with lipidomics determines turnover of molecular GLSs. Rapid and differential turnover of lipid classes and their individual species were observed. Long-chain GSL species showed a more rapid turnover than the very-long-chain species. The analyses revealed different cellular pools of glucosylceramide, lactosylceramide, and globotriaosylceramide. Mass spectrometry analyses of cells labeled with [13C]glucose are a powerful tool to study GSL turnover.