Computational modeling of acrylodan-labeled cAMP dependent protein kinase catalytic subunit unfolding
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- 作者：Aleksei Kuznetsov ; Rait Kivi ; Jaak Jä ; rv ; jaak.jarv@ut.ee" class="auth_mail" title="E-mail the corresponding author
- 关键词：Protein kinase A ; Acrylodan label ; Fluorescence assay ; Protein unfolding ; Intermediate ; molten globule
- 刊名：Computational Biology and Chemistry
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：61
- 期：Complete
- 页码：197-201
- 全文大小：778 K
文摘
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Structure of acrylodan-labeled protein kinase A catalytic subunit was modeled.
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Calculations were made at temperatures from 300 to 700 K to model protein unfolding.
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Positioning of the reporter group was significantly changed during protein unfolding.
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Molten globule formation was observed between the native and unfolded protein structures