Investigation of an outbreak of rickettsial febrile illness in Guatemala, 2007

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• 作者：Marina E. Eremeeva^a ; ¹ ; Elsa Berganza^b ; ^c ; Gloria Suarez^d ; Lorena Gobern^e ; Erica Dueger^d ; Leticia Castillo^e ; Lissette Reyes^f ; ^g ; Mary E. Wikswo^a ; Kyle F. Abramowicz^a ; Gregory A. Dasch^a ; Kim A. Lindblade^h ; ⁱ ; ^kil2@cdc.gov
• 关键词：Amblyomma cajennense ; Spotted fever rickettsiosis ; Spotted fever group rickettsiae ; Guatemala
• 刊名：International Journal of Infectious Diseases
• 出版年：2013
• 出版时间：May, 2013
• 年：2013
• 卷：17
• 期：5
• 页码：e304-e311
• 全文大小：851 K

文摘

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Summary

Objectives

We describe an outbreak of spotted fever group (SFG) rickettsiosis that occurred in 2007 in a farming community in southeastern Guatemala. We identified 17 cases of an acute febrile illness, among which 10, including two fatalities, were confirmed or probable cases of rickettsial disease (case-fatality proportion 12 % ).

Methods

PCR, a microimmunofluorescence assay (IFA), and Western blotting were performed on patient samples, and PCR was performed on ticks.

Results

Using an indirect IFA, seven of 16 (44 % ) ill persons tested had both IgM and IgG antibodies reacting with one or more Rickettsia spp antigens; the other nine (56 % ) had only IgM titers or were seronegative. Antibodies to SFG protein and lipopolysaccharide were detected by Western blotting with antigens of Rickettsia typhi, Rickettsia rickettsii, and Rickettsia akari. Only one sample, from an ill person who died, tested positive by PCR for a SFG Rickettsia. PCR analysis of Amblyomma cajennense ticks from domestic animals in the area detected the presence of SFG Rickettsia DNA in one of 12 ticks collected.

Conclusions

Further studies in Guatemala are warranted to establish the prevalence of rickettsioses and to fully characterize the identity of the etiologic agents and vectors.