Lymphocyte subset distribution and cytokine secretion in third trimester decidua in normal pregnancy and preeclampsia
详细信息 查看全文
- 作者：Wilczynski ; Jacek R. ; Tchó ; rzewski ; Henryk ; Banasik ; Malgorzata ; Glowacka ; Ewa ; Wieczorek ; Artur ; et. al.
- 关键词：HLA ; human leukocyte antigen ; TCR ; T-cell receptor ; IL ; interleukin ; TNF-α ; tumor necrosis factor alpha ; IFN-γ ; interferon gamma ; TGF-β ; transforming growth factor beta ; PE ; preeclampsia ; FITC ; fluorescein isothiocyanate isomer-1 ; RPE ; R-ph
- 刊名：European Journal of Obstetrics and Gynecology and Reproductive Biology
- 出版年：2003
- 出版时间：July 1, 2003
- 年：2003
- 卷：109
- 期：1
- 页码：8-15
- 全文大小：143 K
文摘
Background: Excessive Th1 activity in peripheral blood plays a probable role in the pathogenesis of preeclampsia. The aim of the study was to investigate whether disturbed local immune reactions are also present in decidua. Methods: Flow cytometric analysis of CD3, CD19, CD56/CD16, CD4, CD8, CD4/CD29, CD4/CD45RA, CD4/CD45RO, CD8/CD28, CD3/CD69 lymphocyte subsets isolated from third trimester decidua of pregnants with preeclampsia (n=21) and pregnant controls (n=11) subjected to elective caesarean sections. Spontaneous and phytohemaglutynine stimulated “in vitro” secretion of IL-2, IL-4, IL-6, IL-10, IL-12, IFN-γ and TGF-β by decidual lymphocytes was studied by ELISA. For the statistical significance of differences between the groups the U Mann–Whitney test was performed (confidence interval P<0.05). Results: Preeclamptic patients were characterized with an increased percentage of the CD3−/CD56+CD16+, CD8+/CD28+ and decreased percentage of CD3+, CD19+, CD4+/CD45RA+ lymphocytes. The profile of secreted cytokines shifts in favor of Th1 activity (extremely high IFN-γ and low IL-6 and IL-10 secretion). Decidual IL-12 secretion in preeclamptic patients is decreased compared to controls. Conclusion: Changes in NK and T lymphocyte subsets followed with Th1 cytokine IFN-γ over-activity, could affect local immunoregulatory mechanisms in third trimester decidua of preeclamptic patients.