Differently prepared broccoli meals during in vitro digestion (not colonic) studied.
Endogenous glucoraphanin hydrolysis occurs during digestion mainly in oral phase.
Meal additives did not change the in vitro bioaccessibility of sulforaphane.
Raw/1-min steamed broccoli showed highest sulforaphane formation after digestion.
Endogenous myrosinase was inactivated in the in vitro gastric phase irreversibly.